Effects of Inoculum Amount, Initial pH, and Nicotine Load on the Set-Up of Bioaugmented System with Pseudomonas Sp. HF-1 to Treat Tobacco Wastewater

Table 1

Amount of target gene hsp in the inoculated reactors with different inoculum amount.

Stage	Reactor
	I-1	I-2	I-3	I-4	I-5
	0.55 ± 0.01	1.10 ± 0.03	1.66 ± 0.04	2.21 ± 0.05	2.76 ± 0.07
a	241.69 ± 6.57^a	821.28 ± 9.65^b	298.21 ± 3.95^c	58.77 ± 1.22^d	1.26 ± 0.08^e
b	25.58 ± 0.52^A	40.76 ± 0.50^B	—	—	—

Stage a: from 0 d to 8th d with strain HF-1 inoculation; stage b: from 9th d to 20th d without strain HF-1 inoculation; the inoculum number of reactors I-1 to I-5 was 0.55 ± 0.01, 1.10 ± 0.03, 1.66 ± 0.04, 2.21 ± 0.05, and 2.76 0.07 mg/g (dry weight of strain HF-1/dry weight of activated sludge), respectively.
Different lowercase letters in the same column indicate significant differences at stage a (); different uppercase letters in the same column indicate significant differences at stage b ().