Effects of Inoculum Amount, Initial pH, and Nicotine Load on the Set-Up of Bioaugmented System with Pseudomonas Sp. HF-1 to Treat Tobacco Wastewater

Table 2

Amount of target gene hsp in the inoculated reactors with different initial pH.

Stage	Reactor
	II-1	II-2	II-3	II-4	II-5
	pH 5.0	pH 6.0	pH 7.0	pH 8.0	pH 9.0
a	492.28 ± 72.36^a	873.61 ± 92.95^b	1085.79 ± 15.02^c	10.716 ± 3.83^d	4.30 ± 0.30^d
b	32.95 ± 8.49^A	30.65 ± 4.49^A	47.28 ± 3.33^A	5.71 ± 0.61^B	—

Stage a: from 0 d to 8th d with strain HF-1 inoculation; stage b: from 9th d to 20th d without strain HF-1 inoculation; the initial pH of reactors II-1 to II-5 was 5.0, 6.0, 7.0, 8.0, and 9.0, respectively.
Different lowercase letters in the same column indicate significant differences at stage a (); different uppercase letters in the same column indicate significant differences at stage b ().