Effects of Inoculum Amount, Initial pH, and Nicotine Load on the Set-Up of Bioaugmented System with Pseudomonas Sp. HF-1 to Treat Tobacco Wastewater

Table 3

Amount of target gene hsp in the inoculated reactors with different nicotine load.

Stage	Reactor
	III-1	III-2	III-3	III-4	III-5
	250 mg/L	500 mg/L	800 mg/L	1000 mg/L	1200 mg/L
a	133.38 ± 28.9^a	261.83 ± 39.63^a	847.25 ± 28.97^b	1072.64 ± 27.15^b	1442.08 ± 15.22^c
b	19.56 ± 1.76^A	20.55 ± 2.41^A	36.65 ± 3.71^B	55.73 ± 2.46^C	15.73 ± 1.01^A

Stage a: from 0 d to 8th d with strain HF-1 inoculation; stage b: from 9th d to 20th d without strain HF-1 inoculation; the nicotine load of reactors III-1 to III-5 was 250, 500, 800, 1000, and 1200, respectively.
Different lowercase letters in the same column indicate significant differences at stage a (); different uppercase letters in the same column indicate significant differences at stage b ().