Application of HPLC to Study the Reaction of Free Radicals with Antioxidants and/or Toxins
Figure 6
HPLC chromatograms of p-HBA before (—), after 1 min (–––), and 2 min (- - -) reaction with the hydroxyl radicals. Reaction conditions: concentration of H2O2, 0.03%, Fe2+, 1 mM, and ascorbic acid, 0.1 mM. Chromatographic conditions: column, Eurospher C18 5 μm mm I.D. (Knauer), temperature, 25°C, mobile phase, phosphate buffer with 2 vol.% methanol, flow rate, 1 mL min−1, and fluorescence detection with the excitation/emission wavelengths, 270/358 nm.
