Allelic Variation of Wheat Flour Allergens in a Collection of Wheat Genotypes

Table 2

Wheat allergens accession numbers and primer pairs combinations used for allelic variation detection.

Allergen name	Accession Number	Allergen description	Primer forward (5′-3′)	Primer reverse (5′-3′)
Tri a 12	X89825	Profilin, old name: Tri a profilin	CCAGCACCTCACCTCCGCCGC	ATCGCCGAGCCTCTCCACGACCA
Tri a 21	FN391140	Alpha-beta-gliadin	AGGGCAGCAACAACAACATT	GTTGTTGTTGTTGTAGTTGTGC
Tri a 25	AF286593	Thioredoxin	TGGCTCCAATTTTCGCTGATC	AGAGGCACTACTTGTTGAGC
Tri a 31	AJ278243	Triosephosphate-isomerase	GAACAGGTAGAGAGCATCGT	CCTTCTCTCCAACAAACTCG
Tri a 32	AY304482	1-cys-peroxiredoxin	ATGCCGGGCCTCACCATCGGCGA	CGGGCCGTGGACTCGCTGCTGA
Tri a 33.0101	AJ245879	Serpin	GACGTCCGCCTCTCCATTGCG	AGGTGGGGACAAGAGGCAGT
Tri a 34	FN429985	Glyceraldehyde-3-phosphate-dehydrogenase	AAGTACACCTCAGACGTTAA	ATGGCAAGCTTACCGGTATGT
Tri a 35	FN393741	Dehydrin	ACTACGGTGGAGAGTACGGGCA	CTTGGACCATGTACGACCT
Tri a 36	EU369705	Low molecular weight glutenin GluB3-23	TACAGCAACAACCACCATTTT	GATGACTTCCATTGCGCTC
gstu1C	EU584497	Glutathione transferase	ATGGCGGGCGAGAAGGGCCTGG	AGAAGTACGGCATCGAGTAG