Performance Evaluation of Monolith Based Immobilized Acetylcholinesterase Flow-Through Reactor for Copper(II) Determination with Spectrophotometric Detection

Table 1

Kinetics constants of 𝐾_𝑚 and 𝑉_max for free AChE in solution (A) and AChE immobilized monolith (B).

	𝐾𝑚 (×10^−4 M)	𝑉max (mol L^−1 min^−1)
(A)
Free AChE	2.3	13.6
copper(II) 0.5 mg L^−1	2.3	9.3
copper(II) 1.0 mg L^−1	2.3	7.3
copper(II) 2.0 mg L^−1	2.3	5.5
(B)
Immobilized AChE	2.1	1.9
copper(II) 0.5 mg L^−1	2.1	1.3
copper(II) 1.0 mg L^−1	2.1	0.9
copper(II) 2.0 mg L^−1	2.1	0.7