Performance Evaluation of Monolith Based Immobilized Acetylcholinesterase Flow-Through Reactor for Copper(II) Determination with Spectrophotometric Detection

Table 2

Optimization parameters.

Variable	Studies	Optimum
pH	5–9	8
Concentration of ATChI/mM	0.5–20	5
Concentration of DTNB/mg L^−1	10–120	70
Mixing coil length/cm	10–50	10
Flow rate of ATChI/𝜇L min^−1	10–50	50
Flow rate of DTNB/𝜇L min^−1	10–50	20