Determination of Polar Compounds in Guava Leaves Infusions and Ultrasound Aqueous Extract by HPLC-ESI-MS

Table 2

Quantification (mean ± SD, n = 3) of the compounds identified in guava leaves infusions and ultrasound aqueous extract.

Number	Compounds	Quantification (μg analyte/g leaves)
		AE	I3	I5	I7
1	HHDP glucose isomer	1146 ± 34^c	2256 ± 37^a	2253 ± 27^a	2021 ± 67^b
2	HHDP glucose isomer	228 ± 40^b	441 ± 72^a	368 ± 59^a,b	397 ± 93^a,b
3	HHDP glucose isomer	1424 ± 48^c	1756 ± 39^a	1762 ± 12^a	1546 ± 27^b
4	Gallic acid	719 ± 33^a	240 ± 5^b	260 ± 5^b	254 ± 9^b
5	Prodelphinidin B2 isomer	665 ± 55^a	212 ± 11^b	220 ± 12^b	126 ± 8^c
6	Pedunculagin/casuariin isomer	2405 ± 38^a	573 ± 14^c	688 ± 5^b	533 ± 6^c
7	Prodelphinidin dimer isomer	1768 ± 90^a	444 ± 26^b	467 ± 20^b	335 ± 11^b
8	Gallocatechin	5887 ± 273^a	2419 ± 19^b	2301 ± 43^b,c	1960 ± 77^c
9	Prodelphinidin dimer isomer	5452 ± 308^a	1526 ± 4^b	1688 ± 40^b	1309 ± 34^b
10	Geraniin isomer	1396 ± 47^a	304 ± 1^b	318 ± 12^b	201 ± 8^c
11	Pedunculagin/casuariin isomer	2894 ± 46^a	687 ± 9^c	814 ± 19^b	624 ± 6^c
12	Geraniin isomer	2333 ± 160^a	433 ± 26^b	457 ± 8^b	304 ± 3^b
13	Procyanidin B isomer	17659 ± 785^a	6703 ± 124^b	7106 ± 51^b	6105 ± 34^b
14	Procyanidin B isomer	1751 ± 150^a	398 ± 10^b	413 ± 10^b	321 ± 9^b
15	Tellimagrandin I isomer	728 ± 26^a	141 ± 2^c	184 ± 5^b	129 ± 2^c
16	Catechin	12875 ± 705^a	6127 ± 80^b	5960 ± 19^b	5192 ± 2^b
17	Casuarinin/casuarictin isomer	8725 ± 216^a	482 ± 1^c	859 ± 21^b	590 ± 17^b,c
18	Tellimagrandin I isomer	1492 ± 46^a	186 ± 6^b,c	243 ± 9^b	164 ± 3^c
19	Gallocatechin	5866 ± 362^a	2205 ± 134^b,c	2446 ± 21^b	1896 ± 13^c
20	Myricetin-arabinoside/xylopyranoside isomer	608 ± 18^a	<LOQ	<LOQ	<LOQ
21	Procyanidin B isomer 2	1206 ± 56^a	207 ± 14^b,c	224 ± 7^b	136 ± 6^c
22	Myricetin hexoside isomer	1301 ± 44^a	<LOQ	<LOQ	<LOQ
23	Myricetin hexoside isomer	331 ± 2^a	245 ± 2^c	299 ± 3^b	232 ± 4^d
24	Myricetin-arabinoside/xylopyranoside isomer	639 ± 29^a	<LOQ	<LOQ	<LOQ
25	Quercetin-galloylhexoside isomer	566 ± 20^a	148.3 ± 0.1^b	171 ± 2^b	149 ± 1^b
26	Quercetin-galloylhexoside isomer	452 ± 15^a	97.25 ± 0.02^b	102.3 ± 0.4^b	92.25 ± 0.07^b
27	Myricetin-arabinoside/xylopyranoside isomer	592 ± 24^a	<LOQ	<LOQ	<LOQ
28	Morin	8377 ± 464^a	3235 ± 53^b,c	3676 ± 12^b	3003 ± 18^c
29	Myricetin-arabinoside/xylopyranoside isomer	986 ± 28^a	<LOQ	<LOQ	<LOQ
30	Ellagic acid	4338 ± 234^a	1082 ± 16^b	1367 ± 14^b	1040 ± 9^b
31	Hyperin	7798 ± 280^a	2492 ± 6^c	2891 ± 7^b	2031 ± 18^d
32	Quercetin glucuronide	2293 ± 91^a	1249 ± 19^c	1612 ± 40^b	1132 ± 7^c
33	Isoquercitrin	4408 ± 182^a	1111 ± 19^b,c	1306 ± 5^b	996 ± 3^c
34	Reynoutrin	5849 ± 173^a	1386 ± 21^b,c	1611 ± 20^b	1229 ± 9^c
35	Guajaverin	12843 ± 421^a	3169 ± 40^b,c	3595 ± 4^b	2713 ± 36^c
36	Guavinoside A	1920 ± 40^a	453 ± 3^c	522 ± 1^b	413 ± 2^c
37	Avicularin	13666 ± 421^a	3825 ± 48^b	4183 ± 34^b	3232 ± 25^c
38	Quercitrin	6822 ± 274^a	1825 ± 39^b	2078 ± 24^b	1705 ± 12^b
39	Guavinoside C	2298 ± 40^a	<LOQ	<LOQ	<LOQ
40	Guavinoside B	1456 ± 34^a	370 ± 3^c	423 ± 4^b	328.6 ± 0.1^c
41	Guavinoside A isomer	558 ± 4^a	177 ± 1^b	160 ± 4^c	140.0 ± 0.3^d
42	Prodelphinidin B2 isomer 2	<LOQ	<LOQ	<LOQ	<LOQ
43	Guavinoside B isomer	<LOQ	<LOQ	<LOQ	<LOQ
44	Guavinoside B isomer	<LOQ	nd	nd	nd
45	Guavinoside B isomer	<LOQ	nd	nd	nd
46	Guavin B	460 ± 6^a	34 ± 1^b	32.2 ± 0.2^b	20 ± 1^c
47	Quercetin	408 ± 18^a	nd	nd	nd
48	Naringenin	538 ± 14^c	941 ± 3^a	815 ± 24^b	558 ± 9^c

n.d.: not detected; AE: aqueous extract obtained by ultrasound; I3, I5, and I7: infusion obtained at 3, 5, and 7 minutes of infusion time, respectively.
The different letter in the same line means that the compounds are significantly different (P ≤ 0.05).