Research Article
Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method
Table 1
Validation data for multicomponent spectrometric assay of RF, LC, and LFa.
| | Compound | RF | LC | LF |
| | nm (pH 2.0) | 445 | 356 | 445 | | Molar absorptivity () M−1 cm−1 | 12500 | 10800 | 10400 | | Linearity | | | | | Concentration range (M) | 0.5–5.0 × 10−5 | 0.5–5.0 × 10−5 | 0.5–5.0 × 10−5 | | Slope ×10−4 | 1.250 | 1.080 | 1.040 | | Intercept | 0.0026 | 0.0044 | 0.0035 | | SD of slope | ±0.003 | ±0.003 | ±0.005 | | SE of slope | 0.968 | 1.415 | 1.025 | | Recovery range (%) | 98.5–101.2 | 98.2–100.6 | 98.0–101.8 | | Accuracy (%) ± SD | 100.2 ± 1.21 | 99.8 ± 1.58 | 99.7 ± 1.50 | | RSD (%) | 1.20 | 1.50 | 1.59 | | LOD (M) | 1.195 × 10−6 | 1.251 × 10−6 | 2.160 × 10−6 | | LOQ (M) | 4.183 × 10−6 | 4.405 × 10−6 | 4.420 × 10−6 |
|
|
Values are mean of five determinations.
|
