UAE-HPLC-UV: New Contribution for Fast Determination of Total Isothiocyanates in Brassicaceae Vegetables

Table 1

Multifactorial experimental design 2⁴ for total ITCs extraction.

(a)

Evaluated variables	Levels
	Low	High
() Sample-to-solvent ratio (w/v)	1 : 5	3 : 5
() Homogenization time (min)	2	7
() Use of ultrasound bath (min)	0	5
() Salting out effect (mg mL^−1 NaCl)	0	50

(b)

Run	Extraction condition
	1	1 : 5	5	5	0
2	3 : 5	2	0	50
3	1 : 5	2	0	50
4	1 : 5	5	0	50
5	3 : 5	5	0	50
6	3 : 5	5	5	50
7	3 : 5	2	5	50
8	3 : 5	5	5	0
9	3 : 5	2	0	0
10	1 : 5	5	5	50
11	1 : 5	2	0	0
12	1 : 5	5	0	0
13	3 : 5	2	5	0
14	1 : 5	5	0	0
15	1 : 5	2	5	50
16	1 : 5	2	5	0