UAE-HPLC-UV: New Contribution for Fast Determination of Total Isothiocyanates in Brassicaceae Vegetables

Table 2

Analytical responses values obtained for ITCs concentration for the Central Composite Design arrangement. Analytical responses and predicted values for isothiocyanate production (nmol g⁻¹ w.w.).

(a)

Independent variables	Levels
	−2	−1	0	1	2
: ultrasound time (min)	0.34	2.00	6.00	10.00	11.66
: homogenization time (min)	3.96	5.00	7.50	10.00	11.04

(b)

Run			ITCs production (nmol g^−1 w.w.)
			Observed values	Predicted values
1	−1	−1	252.6	307.2
2	2	0	406.0	386.2
3	−1	1	447.2	447.5
4	1	−1	276.2	286.9
5	0	0	449.6	455.4
6	0	−2	273.6	234.7
7	0	2	446.2	433.1
8	−2	0	444.7	414.9
9	0	0	434.3	455.4
10	1	1	399.5	427.2
11	0	0	453.5	455.4
12	0	0	469.6	455.4
13	0	0	475.6	455.4