UAE-HPLC-UV: New Contribution for Fast Determination of Total Isothiocyanates in Brassicaceae Vegetables

Table 4

Comparison of UAE-HPLC-UV with other analytical methods previously reported for total ITCs determination employing the cyclocondensation reaction.

Method	Bioactive compounds analysed	Sample	LOD	RSD %	Recovery (%)	Reference
SPE-HPLC-DAD	Total isothiocyanate	Human plasma	30 nM		>93.3	Ye et al. 2002 [21]
LLE-HPLC-UV	Total isothiocyanate	Human plasma	20 nM	<6.5	>96.6	Liebes et al. 2001 [26]
Boiling water-GC-MS	Total isothiocyanate	Chinese medicinal herbs and cruciferous vegetables	35 nM		>90.0	Choi et al. 2004 [15]
SPE-HPLC-UV	Total isothiocyanate	Broccoli	25 μmol		>91.2	Bertelli et al. 1998 [20]
UAE-HPLC-UV	Total isothiocyanate	Broccoli, cauliflower, watercress, and cabbage	26 nM	<2.4	>86.0	Proposed work