Antioxidant Peptide Derived from Spirulina maxima Suppresses HIF1α-Induced Invasive Migration of HT1080 Fibrosarcoma Cells

Figure 1

S. maxima peptide decreases CoCl₂-induced invasive migration of HT1080 cells. HT1080 cells were serum starved in DMEM containing 0.1% bovine serum albumin (DMEM-BSA) for 24 h and precultured in the presence or absence of 100 μM CoCl₂ and/or various concentrations of S. maxima peptide for 24 h, transferred onto Matrigel-coated transwell membranes, and further incubated for 16 h under preculture conditions. Migrated cells on the lower surface of the membrane were fixed, stained, and counted. Cells in 9 randomly selected fields were counted in each experiment. Results of at least 3 independent experiments were averaged. All data are presented as mean ± S.D. compared with blank; compared with CoCl₂ control.

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