Development of a New Binary Solvent System Using Ionic Liquids as Additives to Improve Rotenone Extraction Yield from Malaysia Derris sp.
Table 2
Qualitative analysis of rotenone via TLC on varies binary solvent systems.
Binary solvent system
Rotenone migration distance, Ds (cm
Retardation factor
14 hrs
24 hrs
14 hrs
24 hrs
Rotenone standarda
1.80 ± 0.05
2.60 ± 0.03
0.45 ± 0.04
0.65 ± 0.03
Control (acetone)
2.03 ± 0.06
2.60 ± 0.01
0.51 ± 0.02
0.65 ± 0.04
BMIM Cl + acetone
1.93 ± 0.06
2.20 ± 0.02
0.49 ± 0.01
0.55 ± 0.01
BMIM Oac + acetone
1.53 ± 0.06
1.40 ± 0.04
0.39 ± 0.01
0.35 ± 0.01
BMIM NTF2 + acetone
3.20 ± 0.01
2.17 ± 0.06
0.80 ± 0.01
0.54 ± 0.01
BMIM OTF + acetone
3.20 ± 0.08
1.70 ± 0.07
0.80 ± 0.08
0.43 ± 0.06
BMP Cl + acetone
3.10 ± 0.00
2.93 ± 0.06
0.78 ± 0.00
0.74 ± 0.01
Rotenone standard was prepared in acetone + ILs. bSpotting volume (TLC spotting capillary tubes): ~10 L. Sample size: ~25 to 42 g rotenone/spot. Error bars represent the standard deviation (SD) of triplicates ( = 3).
