Mango Fruit Extracts Differentially Affect Proliferation and Intracellular Calcium Signalling in MCF-7 Human Breast Cancer Cells

Figure 2

ERK activation in MCF-7 tumour cells. The phosphorylation of ERK (P-ERK) ((a) and (c)) and normalisation of total ERK (T-ERK) ((b) and (d)) in lysates from cells treated with peel and flesh extracts from mango cultivars IW, KP, and NDM were determined by immunoblotting. Bar graphs indicate quantification of at least 3 separate blots using Quantity One software. Data are expressed as mean ± SE. , significant difference from PMA-treated controls ().

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