Monitoring Antibiotic Residues and Corresponding Antibiotic Resistance Genes in an Agroecosystem

Table 3

Polymerase chain-reaction (PCR) primers for tetracycline- (TC-) and sulfonamide- (SA-) resistant gene classes.

Gene	Primer	Sequences	Annealing temp. (°C)	Amplicon size (bp)
tet(S)	tetS-FW	GAAAGCTTACTATACAGTAGC	50	169
	tetS-RV	AGGAGTATCTACAATATTTAC
tet(T)	tetT-FW	AAGGTTTATTATATAAAAGTG	46	169
	tetT-RV	AGGTGTATCTATGATATTTAC
otr(A)	otrA-FW	GGCATYCTGGCCCACGT	66	212
	otrA-RV	CCCGGGGTGTCGTASAGG
sul(I)	sulI-FW	CGCACCGGAAACATCGCTGCAC	55.9	163
	sulI-RV	TGAAGTTCCGCCGCAAGGCTCG
sul(II)	sulII-FW	TCCGGTGGAGGCCGGTATCTGG	60.8	191
	sulII-RV	CGGGAATGCCATCTGCCTTGAG
sul(III)	sulIII-FW	TCCGTTCAGCGAATTGGTGCAG	60	128
	sulIII-RV	TTCGTTCACGCCTTACACCAGC
sul(A)	sulA-FW	TCTTGAGCAAGCACTCCAGCAG	60	229
	sulA-RV	TCCAGCCTTAGCAACCACATGG

Forward.
Reverse.