Study of Synthesis, Characterization, DFT, and In Vitro Biological Activity of Cu(II), Co(II), and Fe(II) Metal Complexes Based on Heterocyclic Azo Pyrazole Dye Ligand
Table 8
DNA fragmentation of (a) azo ligand and its (b) Cu(II), (c) Co(II), and (d) Fe(II) complexes at (0.1, 1 μg/mL) on HepG2 cells.
(a)
Groups
DNA fragmentation%
SD
P1 Student’s t-test
P2 Student’s t-test
Test of significance
HepG2 treated with 0.1 μg/mL
14.100228
1.255707
0.001
<0.001
ANOVA
F = 560.109
HepG2 treated with 1 μg/mL
41.332428
1.830609
<0.001
Control group
7.004727
0.574902
(b)
Groups
DNA fragmentation%
SD
P1 Student’s t-test
P2 Student’s t-test
Test of significance
HepG2 treated with 0.1 μg/mL
14.444136
1.286334
0.001
<0.001
ANOVA
F = 560.109
HepG2 treated with 1 μg/mL
42.340536
1.875258
<0.0010
Control group
7.175574
0.588924
(c)
Groups
DNA fragmentation%
SD
P1 Student’s t-test
P2 Student’s t-test
Test of significance
HepG2 treated with 0.1 μg/mL
15.47586
1.378215
0.001
<0.001
ANOVA
F = 560.109
HepG2 treated with 1 μg/mL
45.36486
2.009205
<0.0010
Control group
7.688115
0.63099
(d)
Groups
DNA fragmentation%
SD
P1 Student’s t-test
P2 Student’s t-test
Test of significance
HepG2 treated with 0.1 μg/mL
14.90268
1.32717
0.001
<0.001
ANOVA
F = 560.109
HepG2 treated with 1 μg/mL
43.68468
1.93479
<0.0010
Control group
7.40337
0.60762
P1: comparison of both study groups versus the control group. P2: comparison between both the study groups; ∗P is significant at <0.05.
