Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting

Table 2

PCR primer set of genes analyzed to assess DNA amplificability and integrity.

Region	Forward primer	Reverse primer	Product size (bp)	Annealing temperature (°C)
KRAS	ACTGAATATCTTGTGGTAGTTGGAGCT	TCAAAGAATGGTCCTGGACC	157	54
TP53 ex 6	CTGGAGAGACGACAGGGCTG	GACAACCACCCTTAACCCCTC	219	60
TP53 ex 7	CTTGCCACAGGTCTCCCCAA	TCAGCGGCAAGCAGAGGCTG	233	60
TP53 ex 8	CGCCCGCCGCGCCCCGCGCCCGTCCCG CCGCCCCCGCCCGACTGCCTCTTGCTTCTC TTTTCC	AATCTGAGGCATAACTGCACCC	271	62
NRAS	GTCACTTTAAGAACCAAATGG	GAGAGACAGGATCAGGTCA	288	54
NRAS	CTTGGCAATAGCATTGTATTC	GATTCAGAACACAAAGATCATC	315	54
TP53 ex 5	GCTGCCGTGTTCCAGTTGC	TCAGTGAGGAATCAGAGGCC	330	60
KRAS	GTTGAGTTGTATATAACACC	ATTATATGCATGGCATTAGC	343	54