BAY 61-3606, CDKi, and Sodium Butyrate Treatments Modulate p53 Protein Level and Its Site-Specific Phosphorylation in Human Vestibular Schwannomas In Vitro

Figure 1

(a) Representative Western blot analysis of the effect of Na-Bu, CDKi, and BAY 61-3606 on the level of p53 protein in VS tumor sample in vitro 48 hours after treatment. The 53 kDa p53 band and the 48 kDa γ-tubulin bands are marked as indicated on the left. The WI38 was used as an additional control. VS18 is a tumor sample from a 55-year-old female patient; VS1 is a tumor sample from 26-year-old male patient. The lanes are as marked above: UT, untreated; Na-Bu, sodium butyrate treated; CDKi, CDKi treated; BAY 61, BAY 61-3606 treated. (b) Histogram of the combined data from VS tumor samples showing level of p53 and various phosphorylated forms of p53 proteins 48 hours after treatment. Total p53 and Thr55 bars represent data for 20 VS tumor samples. Ser392 (Y) and Ser392 (O) represent combined data from 10 young (35 years or younger) and 10 old (above 35 years old) patients, respectively. Ser315 (Y) and Ser315 (O) represent the same young and old patients as mentioned for Ser392. Ser15 (Y) data is from young patients only. The standard deviations are as indicated in the chart; .

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