BAY 61-3606, CDKi, and Sodium Butyrate Treatments Modulate p53 Protein Level and Its Site-Specific Phosphorylation in Human Vestibular Schwannomas In Vitro

Figure 3

Western blot analysis of the effect of Na-Bu, CDKi, and BAY 61-3606 on the MDM2/p53 ratio in VS tumor sample 48 hours after treatment in vitro. The Western blot analysis was done after immunoprecipitation with p53 antibody as given under Methods. The 53 kDa p53 band and the 90 kDa MDM2 bands are as given on the left. The levels of p53 and MDM2 in the tumor lysates are in the left panel. The MDM2/p53 ratios after immunoprecipitation are given on the right panel and the actual ratios are given below the corresponding lanes. The MDM2/p53 ratio of WI38 was taken as 1.0 and used as a control. (a) shows data for VS sample from young patient (26 years old); (b) shows data for VS sample from old patient (55 years old). Lane U, untreated; Lane B, Na-Bu treated; Lane C, CDKi treated; Lane S, BAY 61-3606 treated.

(a)

(b)