miR-204 Shifts the Epithelial to Mesenchymal Transition in Concert with the Transcription Factors RUNX2, ETS1, and cMYB in Prostate Cancer Cell Line Model

Figure 1

Luciferase reporter assay validation of syn-hsa-miR204 and anti-hsa-miR204 efficiency. Luminescence readings (arbitrary units) of miR-204 seed specific LightSwitch 3′UTR GoClone Luciferase reporter cotransfected with MiSrcipt Inhibitor Negative Control (targeting only AllStars Negative siRNA) and miR-204 mimic, or miR-204 inhibitor, normalized to AllStars Negative siRNA cotransfection. miR-204 mimic binding to the reporter 3′UTR results in decreased Luciferase signal. Effect of miR-204 on EMT. AllStars Negative siRNA, MiSrcipt Inhibitor Negative Control, miR-204 mimic, or miR-204 inhibitor transfected LNCaP, PC3, VCaP, and NCI-H660 cell lines were harvested for total RNA. qPCR was performed for SLUG, ECDH, NCDH, VEGFA, SOX9, and the relative gene expression (Pfaffl, ), normalized to PGK1, was assessed for LNCaP, PC3, VCaP, and NCI-H660 cell lines. , . Error bars represent s.d., independent biological repeats.