Research Article

miR-204 Shifts the Epithelial to Mesenchymal Transition in Concert with the Transcription Factors RUNX2, ETS1, and cMYB in Prostate Cancer Cell Line Model

Figure 2

E-Cadherin has differential splicing variant expression in LNCaP cells compared to all other cell lines: PC3, VCaP, and NCI-H660. Dissociation and amplification curves (Stratagene MX3005P) of ECDH specific primers amplified product in total RNA from LNCaP, PC3, VCaP, and NCI-H660 cells (AllStars Negative siRNA, MiSrcipt Inhibitor Negative Control, miR-204 mimic, or miR-204 inhibitor). Different Tm in dissociation curves are highlighted using vertical black bars.