miR-204 Shifts the Epithelial to Mesenchymal Transition in Concert with the Transcription Factors RUNX2, ETS1, and cMYB in Prostate Cancer Cell Line Model

Figure 5

Transcription factors cMYB, RUNX2, and ETS1 siRNA knockdown validation. qPCR validation. Cell lines LNCaP, PC3, VCaP, and NCI H660 were analyzed for cMYB, RUNX2, and ETS1 mRNA expression by RT-qPCR after specific siRNA or AllStars Negative siRNA transfection. qPCR data are represented as a relative fold change normalized to nontreated cells (100%). FCS validation. Cell lines LNCaP, PC3, VCaP, and NCI H660 were analyzed for cMYB, RUNX2, and ETS1 protein expression after specific siRNA or AllStars Negative siRNA transfection using FCS. Expression is presented as FCS overlay histograms of isotype control, AllStars Negative siRNA, and specific siRNA transfected cells. MFI bar chart represents the mean fluorescent intensity index of each FCS overlay histogram, normalized to MFI of AllStars Negative siRNA transfected cells (100%).