miR-204 Shifts the Epithelial to Mesenchymal Transition in Concert with the Transcription Factors RUNX2, ETS1, and cMYB in Prostate Cancer Cell Line Model

Figure 6

Transcription factors cMYB, RUNX2, and ETS1 are EMT regulators. LNCaP, PC3, VCaP, and NCI-H660 cells transfected for 72 h using either AllStars Negative siRNA or siRNA specific for RUNX2, ETS1, or cMYB, were all harvested for total RNA. qPCR was performed for SLUG, ECDH, NCDH, VEGFA, SOX9, and the relative gene expression represented as log₁₀ change (Pfaffl, ), normalized to PGK1, was assessed for LNCaP, PC3, VCaP, and NCI-H660 cell lines. , . Error bars represent s.d., independent biological repeats.