miR-204 Shifts the Epithelial to Mesenchymal Transition in Concert with the Transcription Factors RUNX2, ETS1, and cMYB in Prostate Cancer Cell Line Model

Figure 7

Total demethylation suppresses E-cadherin and upregulates N-cadherin and SLUG. 5-AzaC treated or nontreated LNCaP, PC3, VCaP, and NCI-H660 cells were harvested for total RNA. qPCR was performed for SLUG, ECDH, NCDH, VEGFA, SOX9, and the relative gene expression represented as log₁₀ change (Pfaffl, ), normalized to PGK1, was assessed for LNCaP, PC3, VCaP, and NCI-H660 cell lines. , . Error bars represent s.d., independent biological repeats.