Table 2: Tyrosine hydroxylase (TH) in brain and peripheral organs in the rat 3 days after intravenous injection of gene therapy with TH expression plasmids driven by either the SV40 promoter (clone 877) or the Gfap promoter (clone 951), respectively.
Organs
Saline (pmol/hr/mgp)
TfRMAb-THL/877 (pmol/hr/mgp)
TfRMAb-THL/951 (pmol/hr/mgp)
Ipsilateral striatum
128 ± 27
5177 ± 446*
5536 ± 395*
Contralateral striatum
6445 ± 523
5832 ± 391
5713 ± 577
Ipsilateral cortex
176 ± 30
132 ± 16
184 ± 38
Contralateral cortex
150 ± 36
150 ± 24
135 ± 25
Heart
29 ± 3
45 ± 8
31 ± 3
Liver
13 ± 2
130 ± 28**
18 ± 6
Lung
42 ± 13
74 ± 22
30 ± 6
Kidney
24 ± 2
35 ± 5
31 ± 8
*
difference from saline group (ANOVA with Bonferroni correction; rats per group). Rats were lesioned with intracerebral injections of 6-hydroxydopamine; 3 weeks after toxin injection, the rats were tested for apomorphine-induced rotation behavior; those rats testing positively to apomorphine were selected for gene therapy, which was administered intravenously 4 weeks after toxin administration; all animals were euthanized 3 days after gene administration. From [30].