Optimization of Liposomal Lipid Composition for a New, Reactive Sulfur Donor, and In Vivo Efficacy Studies on Mice to Antagonize Cyanide Intoxication

Table 2

Rh-load optimization with and without DOTAP.

Composition of liposomes	Original Rh concentration (mg/mL)	% of Rh incorporation determined by Bradford
POPC : Chol : PEG-PE-2000 = 56.9 : 38: 5.1	1.00	16.6
POPC : Chol : PEG-PE-2000 = 56.9 : 38: 5.1	0.25	26.9
POPC : Chol : PEG-PE-2000 : DOTAP = 82.7 : 9.2 : 5.1 : 3.0	1.67	18.2
POPC : Chol : PEG-PE-2000 : DOTAP = 82.7 : 9.2 : 5.1 : 3.0	0.50	55.8
POPC : Chol : PEG-PE-2000 : DOTAP = 82.7 : 9.2 : 5.1 : 3.0	0.25	74.0
POPC : Chol : PEG-PE-2000 : DOTAP = 82.7 : 9.2 : 5.1 : 3.0	1.00	52.9