Preparation of a Nanoscaled Poly(vinyl alcohol)/Hydroxyapatite/DNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery

Table 1

DLS measurement of PVA/DNA and PVA/HAp/DNA complexes at various PVA and HAp concentrations. DNA conc.: 0.0025 w/v%.

HAp (%)	Average diameter (nm) (PDI) PVA (%)
	0.001	0.005	0.01	0.05	0.1
0	306 ± 7 (0.08 ± 0.02)	325 ± 8 (0.13 ± 0.02)	360 ± 11 (0.16 ± 0.02)	525 ± 16 (0.07 ± 0.03)	649 ± 65 (0.11 ± 0.02)
0.00001	346 ± 4 (0.12 ± 0.02)	393 ± 8 (0.13 ± 0.02)	355 ± 14 (0.20 ± 0.02)	602 ± 17 (0.08 ± 0.03)	756 ± 17 (0.12 ± 0.03)
0.0001	358 ± 3 (0.11 ± 0.03)	397 ± 8 (0.10 ± 0.03)	390 ± 8 (0.19 ± 0.01)	649 ± 21 (0.12 ± 0.03)	770 ± 20 (0.08 ± 0.03)
0.0005		571 ± 34 (0.24 ± 0.13)	572 ± 27 (0.15 ± 0.03)	689 ± 17 (0.05 ± 0.03)	1090 ± 44 (0.21 ± 0.03)
0.001	578 ± 42 (0.278 ± 0.04)	684 ± 14 (0.11 ± 0.21)	785 ± 21 (0.11 ± 0.02)	1080 ± 21 (0.25 ± 0.02)	1310 ± 59 (0.23 ± 0.03)