Effects of Atelocollagen Formulation Containing Oligonucleotide on Endothelial Permeability
Figure 2
Effects of the AC formulation on endothelial paracellular flux. (a) Increase in dextran permeation induced by the AC formulation. One hour after the addition of oligodeoxynucleotides (ODN), atelocollagen (AC), or the AC formulation (AC + ODN) to the inner chambers, Texas red-conjugated dextran (MW: 40,000) was added to the inner chambers. One hour later, the dextran concentration of the medium in the outer chambers was determined. (b) Enhanced paracellular transport of atelocollagen. 0.1% w/v of Alexa Fluor 594 conjugated bovine serum albumin (BSA; MW: 66 kDa), 0.1% w/v of fluorescein conjugated dextran (dextran; MW: 70 kDa), or rhodamine red-conjugated atelocollagen (0.03% w/v: atelocollagen (Low); 0.1 % w/v: atelocollagen (High); MW of atelocollagen: 300 kDa) with 5 μM of ODN was added to the inner chambers. The solute concentrations of the outer chambers were analyzed by measuring their fluorescence at 1 and 2 hours after the start of the experiment. TER at 2 hours is represented as a percentage value compared to that observed at the start of the experiment.
