Effects of Atelocollagen Formulation Containing Oligonucleotide on Endothelial Permeability

Figure 4

Effects of atelocollagen combined with ODN on intercellular formation. (a) Images of actin, VE-cadherin with actin, ZO-1, and claudin-5 in the endothelial cells of the blank control (upper panels) and the cells treated with the AC formulation (lower panels). Treatment with the AC formulation (AC + ODN) induced changes in cell morphology associated with stress fiber formation (arrowhead), intercellular gap formation (G), and the loss of VE-cadherin and ZO-1. (b) Western blot analysis of actin, VE-cadherin, ZO-1, and claudin-5. The whole cell extract obtained from the cells was separated by SDS-PAGE and immunoblotted with the corresponding antibodies. BC: blank control, ODN: treated with ODN alone, AC: treated with atelocollagen alone, AC + ODN: treated with the AC formulation.