Cationic Albumin Nanoparticles for Enhanced Drug Delivery to Treat Breast Cancer: Preparation and In Vitro Assessment
Figure 3
Cellular uptake of PEI-enhanced nanoparticles was assessed with respect to different amounts of PEI used for coating (mean ± S.D., ). PEI-enhanced HSA nanoparticles were prepared using an ethanol desolvation technique with 20 mg/mL HSA. The nanoparticles were composed of 5% tetramethylrhodamine-conjugated BSA, and the cellular uptake was observed under a fluorescence microscope (TE2000-U, Nikon; USA). (a) Percentage of cellular uptake with nanoparticles prepared using 0, 10, 20, and 30 μg of PEI per mg of HSA. Varying quantities of nanoparticle preparations were added to the cells: 50, 100, and 200 μL. Fluorescence images of cellular uptake of different HSA nanoparticle preparations, consisting of tetramethylrhodamine-conjugated BSA, are shown; (b) uncoated HSA nanoparticles, (c) 10 μg and (d) 30 μg of PEI added per mg of HSA to form PEI-enhanced HSA nanoparticles. Corresponding bright field images are illustrated below (e, f, and g).
