Cationic Albumin Nanoparticles for Enhanced Drug Delivery to Treat Breast Cancer: Preparation and In Vitro Assessment

Table 2

Effect of incubation time for PEI coating and stirring speed during the desolvation step on the physical characteristics of drug-loaded PEI-enhanced HSA nanoparticles, prepared with 20 mg/mL HSA and 30 μg of PEI added per mg of HSA (mean ± S.D., 𝑛=3).

Time of incubation with PEI (hrs)	Stirring speed (rpm)	Particle size (nm)	Zeta potential (mV)
4	250	412.76 ± 12.7	8.94 ± 0.12
	500	248.43 ± 1.7	7.20 ± 0.19
	1000	130.47 ± 11.3	4.24 ± 0.08
8	250	362.77 ± 0.65	17.4 ± 0.36
	500	218.57 ± 15.9	19.14 ± 0.51
	1000	100.73 ± 3.93	18.39 ± 0.27
12	250	332.67 ± 16.2	16.13 ± 0.91
	500	205.17 ± 8.16	10.99 ± 0.71
	1000	111.53 ± 4.72	13.73 ± 0.36