Research Article

Active Targeting to Osteosarcoma Cells and Apoptotic Cell Death Induction by the Novel Lectin Eucheuma serra Agglutinin Isolated from a Marine Red Alga

Figure 4

Specific binding of ESA to either OST cells or LM8 cells, as measured by using a flow cytometer. The cells were cultured with 10% FBS D-MEM containing FITC-labeled ESA at 37°C in a humidified atmosphere of 5% CO2. After incubation for 0, 3, 6, 9, 12, and 24 hours, the cells were washed with PBS, followed by evaluation of the amount of ESA which was bound to the cells.
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