Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis
Figure 2
Immunofluorescence image and binding assay for M/D-CTX-Fcs using A172 cells. (a) The M/D-CTX-Fcs attached to cell surfaces at 4°C (upper). Fifteen minutes incubation at 37°C promoted the internalization of M/D-CTX-Fcs into cells (lower). The cells were stained with anti-human IgG antibody labeled with FITC, anti-EEA1 antibody and anti-rabbit IgG antibody labeled with alexa-555, and DAPI. Left: fluorescence image; right; composite image. M: M-CTX-Fc; D: D-CTX-Fc. Bars = 10 μm. (b) The binding ability of M/D-CTX-Fcs was evaluated by EIA. A172 cells were fixed on EIA plates and exposed to M/D-CTX-Fcs. The affinity of M-CTX-Fc for cell surfaces was higher than that of D-CTX-Fc.
