Targeted Antiepidermal Growth Factor Receptor (Cetuximab) Immunoliposomes Enhance Cellular Uptake In Vitro and Exhibit Increased Accumulation in an Intracranial Model of Glioblastoma Multiforme

Figure 7

Distribution of green DiO-containing α-hEGFR-IL’s (A–C) and hIgG-IL’s (D–F) in the U87 mg intracranial tumor xenograft co-detected with laminin using a red fluorescent antibody to detect capillaries (asterisks). To enhance the visualization of the green fluorescence emitting DiO-containing liposomes, the sections were additionally incubated with Alexa Fluor 488-conjugated anti-human immunoglobulins. (C), (F) When examined at large magnification (marked areas in (B) and (E)), it is evident that the presence of α-hEGFR-IL’s is higher within U87 mg cells compared with that of hIgG-IL’s (compare 7(C) with 7(F)). Furthermore, it is evident that the green liposomes are present in cells non-labeled in red (arrows). Cellular nuclei identified with DAPI (blue). Scale bar = 50 μm.