513950.fig.002a
(a)
513950.fig.002b
(b)
513950.fig.002c
(c)
513950.fig.002d
(d)
513950.fig.002e
(e)
513950.fig.002f
(f)
Figure 2: Phase contrast microscopy images of IEC-6 cells cultured with Hoechst 33342. (a), (b), and (c) show cultures grown in the absence of Hoechst 33342 (a), or in the presence of 1 (b), or 5 μg/mL Hoechst 33342 (c) for 1 day. Note that many cells were detached when 5 μg/mL of dye was used. (d), (e), and (f) show cultures grown in the presence of 1 μg/mL Hoechst 33342 for 4 (d), 7 (e), or 12 days (f). Note that bundle-like structures (indicated by arrows) were observed in (e) and (f).