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Figure 4: (a) Dose response relationship between Hoechst 33342 and fluorescence intensity in the presence or absence of 50 μM verapamil in IEC-6 cells, (b) Hoechst 33342 dose response for fluorescence intensity in IU-937 cells, and (c) FACS analysis of IEC-6 cells incubated with 0, 10, 100, or 1000 ng/mL Hoechst 33342. Note that the small peak (indicated by an arrow) is also observed at 100 ng/mL of dye. (d) FACS analysis of U-937 cells incubated with 0, 10, 100, or 1000 ng/mL Hoechst 33342.