513950.fig.005a
(a)
513950.fig.005b
(b)
513950.fig.005c
(c)
513950.fig.005d
(d)
513950.fig.005e
(e)
Figure 5: Effect of fixation, dehydration, and freezing of Hoechst 33342-stained IEC-6 cells on fluorescence intensity. IEC-6 cells stained with 100 ng/mL Hoechst 33342 were observed by both phase contrast and fluorescent microscopy before ((a), (b)) and after treatment ((c), (d)). Fluorescence intensity was also measured before and after treatment (e). Significant differences were detected as shown by an asterisk ( ).