513950.fig.008a
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513950.fig.008b
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513950.fig.008c
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513950.fig.008d
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Figure 8: (a) Fluorescence intensity of U-937 cells was analyzed after staining with serial concentrations of Hoechst 33342 using FACS Aria II. The segments P1, P2, P3, or P4 correspond to the range of fluorescence intensity at 0, 10, 100, or 1000 ng/mL Hoechst 33342, respectively. (b) FACS analysis of isolated peritoneal macrophages from mice injected with the control (left) or Hoechst 33342-incorporated particles (middle and right). The cells were isolated 20 (middle) or 60 hours (right and left) after injection. P1, P2, P3, and P4 indicate the above range of fluorescence intensity at 0, 10, 100 or 1000 ng/mL Hoechst 33342, respectively. (c) The ratio of isolated peritoneal macrophages with fluorescence intensity that fell into segments P1, P2, P3 or P4. (d) Estimated mean concentration of Hoechst 33342 to which the peritoneal macrophages were exposed. The standard curve was generated using U-937 cells exposed to 10, 100, or 1000 ng/mL Hoechst 33342.