Enhanced Dendritic Cell-Mediated Antigen-Specific CD4+ T Cell Responses: IFN-Gamma Aids TLR Stimulation

Figure 1

IFN-gamma enhances DC maturation with or without TLR ligands in vitro. C57BL/6 bone marrow cells were cultured with GM-CSF to generate bone marrow derived DCs. At days 4-5, cells were preconditioned with IFN-gamma for 2 h (solid line) or no IFN-gamma (dotted line), followed by LPS (TLR4 ligand) or CpG (TLR9 ligand) stimulation for 16 h. Cells were harvested and labelled with fluorescent antibodies. Live CD11c+ cells were gated and analyzed for CD40, CD80, CD86, and MHC-class II expression. Data shown are representative of at least two experiments. The shaded area represents cells stained with the respective secondary antibody.