Enhanced Dendritic Cell-Mediated Antigen-Specific CD4+ T Cell Responses: IFN-Gamma Aids TLR Stimulation
Figure 3
IFN-gamma enhances DC costimulation only when the TLR ligand is present. Days 4-5 bone marrow cultures preconditioned with IFN-gamma (black symbols) or no IFN-gamma (open symbols) for 2 h was stimulated with LPS (TLR4 ligand) or zymosan (TLR2 ligand) for 16 h. DCs were purified via the AutoMacs system as described in Section 2. Titrated bone marrow-derived DCs () were incubated with CD4 T cells in quadruplicates in 96-well plates that were precoated with anti-CD3. Cell proliferation was monitored from day 2 to day 7. Proliferation kinetics was exemplified when DCs were seeded at (a, b, c). As proliferation in general peaked at day 5, it was compared across DC titrations (d, e, f). Data shown are representative of two separate experiments. in LPS and zymosan groups at all points except time point 2 days, based on quadruplicate values.
