Enhanced Dendritic Cell-Mediated Antigen-Specific CD4+ T Cell Responses: IFN-Gamma Aids TLR Stimulation
Figure 4
IFN-gamma enhances DC antigen presentation via MHC-class II, only in the presence of a TLR stimulus. Day 4 bone marrow cultures preconditioned with IFN-gamma for 2 h were pulsed with OVA in the presence of LPS (TLR4 ligand) or zymosan (TLR2 ligand) for 3 h. DCs were purified as described in Section 2. Titrated bone marrow-derived DCs () were incubated with CD4 T cells in quadruplicates in 96-well plates. Cell proliferation was monitored from day 1 to day 5. Proliferation kinetics was exemplified when DCs were seeded at (a, b, c). Peak proliferation at day 3 was compared across DC titrations (d, e, f). Data shown are representative of two separate experiments. in all LPS and zymosan groups at all points, based on quadruplicate values.