Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase
Figure 1
Contour plot for factorial studies. (a) The effect of various levels of phosphatidylcholine and cholesterol on percentage protein encapsulation when the level of the other variables was maintained at their lowest level. (b) The effect of various levels of phosphatidylcholine and cholesterol on percentage protein encapsulation when the level of the other variables was maintained at their highest level. (c) The effect of various levels of temperature (during film formation) and speed of rotation of the flask (rpm) on percentage protein encapsulation when the level of the other variables was maintained at their lowest level. (d) The effect of various levels of temperature (during film formation) and speed of rotation of the flask (rpm) on percentage protein encapsulation when the level of the other variables was maintained at their highest level.
