In Vivo siRNA Delivery and Rebound of Renal LRP2 in Mice

Figure 7

siLRP2 did not reduce renal megalin protein expression 3 h or 12 h after a single dose. (a) Immunohistochemistry (IHC) of a control mouse. (b) IHC 3 h after administration of siLRP2. (c) IHC 12 h after administration of siLRP2. (d) IHC of a control mouse after 3.5 days of PBS vehicle. (e) IHC 3 h after a final seventh administration of siLRP2. (f) IHC staining density as quantified showed no significant difference; IHC staining density by visual scoring also revealed no significant difference. (g) Immunoblot densitometry of megalin in mice receiving siLRP2. (h) Immunoblots of megalin and actin for mice. = 4 per group for all analyses. Control mice for the 3 h and 12 h time points were the same and sacrificed 3 h after vehicle administration (all three groups were sacrificed the same day). For IHC, 10 images were scored for each mouse. All images 20x, measurement bar is 50 μm.

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