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Experimental Diabesity Research
Volume 5 (2004), Issue 3, Pages 227-235
http://dx.doi.org/10.1080/15438600490489793

Effect of High Glucose Concentration on Collagen Synthesis and Cholesterol Level in the Phenotypic Modulation of Aortic Cultured Smooth Muscle Cells of Sand Rat (Psammomys obesus)

1Nutrition and Metabolism Physiology (LBPO), P.O. Box 32, USTHB, Algiers, Algeria
2Extracellular Matrix (LBCM), FSB, USTHB, Algiers, Algeria
3EA 3740 Université Claude Bernard Lyon-1, Faculté de Médecine RTH Laënnec, Lyon Cedex, France
4Central Hospital of the Army, Ain Naâdja, Algiers, Algeria

Received 26 May 2003; Accepted 30 May 2004

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To simulate diabetic conditions, the effects of high glucose concentration on collagen synthesis and cholesterol level in cultured aortic smooth muscle cells of Psammomys were investigated. For collagen biosynthesis, smooth muscle cells (SMCs) were incubated in synthetic proliferative phase and in postconfluent phase with H3-proline. Cellular cholesterol was determined by enzymatic method. Under high glucose concentration, the results showed morphological modifications characterized by morphometric cellular, nuclear, and nucleolar changes. In biochemical studies, the authors observed an increase of free and esterified cellular cholesterol as well as of total proteins, collagen biosynthesis, and α1 (I+III) and α2 (I) chains of collagen contained in the SMCs and in the extracellular matrix. These results showed the sensitivity of Psammomys aortic SMCs to high glucose concentration and would constitute an interesting cellular model to study atherosclerosis pathogeny in experimental diabetes.