Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation

Figure 4

Characterisation of recproIAPP, recN+IAPP, recIAPP, and recIAPP+C. (a) A cartoon showing the amino acid sequences of recproIAPP, recN+IAPP, recIAPP, and recIAPP+C peptides. N-terminally, a fragment consisting of 10 residues originating from the GST-tag is present (white box). (b) Silver stained tricine SDS-PAGE of recproIAPP, recN+IAPP, recIAPP, and recIAPP+C. Bands were removed from the tricine gel, trypsinised and analysed by electrospray ionisation tandem mass spectrometry.

(a)

(b)