Glucose Regulation of Thrombospondin and Its Role in the Modulation of Smooth Muscle Cell Proliferation
Figure 5
TSP-1 protein levels regulate β3 phosphorylation. SMCs expressing the TSP-1 or a scrambled (Scr) RNAi construct were grown to confluency in DMEM containing 25 mM glucose and then incubated overnight in SFM with 25 mM glucose prior to treatment with 4N1K or 4NGG (1 g/mL) or TSP-1 (0.5 and 1 g/mL) for 6 hours prior to lysis. The graph shows the mean fold decrease, of three independent experiments. when the level in TSP-1 RNAi cultures is compared with controls.
