Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes
Figure 2
Effect of high osmolarity on (a, c) protein expression and (b) activity of AR in podocytes cultured in normal (NG) and high (HG) glucose. Protein expression was quantified by densitometry of immunoblot bands (a) and by flow cytometry (c) as indicated in Materials and Methods. Data are shown as percent changes of respective normoosmolar (Nosm) control values at the indicated time points (a, b) or as a mean fluorescence index, MFI (c). Results are expressed as means ± SE from 3–5 independent experiments, *, ** versus Nosm cells from respective groups.
