Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes

Figure 4

Effect of high glucose on (a, c) protein expression and (b) activity of AR in podocytes cultured in normo- (Nosm) and high- (Hosm) osmotic medium. Protein expression was quantified by densitometry of immunoblot bands (a) and by flow cytometry (c) as indicated in Materials and Methods. Data are shown as percent changes of respective normoglycemic (NG) control values at the indicated time points (a, b) or as a mean fluorescence index, MFI (c). Results are expressed as means ± SE from 3–5 independent experiments, *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 versus NG cells from respective groups.

(a)

(b)

(c)