Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes

Table 2

PCR conditions for aldose reductase and β-actin genes. Previously published primer sequences were verified by NCBI BLAST. Amplification was conducted for 26 cycles.

Gene	Sequence of primers (5′ to 3′)	Amplicon (bp)	Annealing temp. (°C)
Aldose reductase	Sense: CCCAGGTGTACCAGAATGAGA	580	53.1
	Antisense: TGGCTGCAATTGCTTTGATCC
β-actin	Sense: CCGTAAAGACCTCTATGCCA	299	50.8
	Antisense: AAGAAAGGGTGTAAAACGCA